Journal: Advanced Science

Article Title: A Self‐Assembling LYTAC Mediates CTGF Degradation and Remodels Inflammatory Tumor Microenvironment for Triple‐Negative Breast Cancer Therapy

doi: 10.1002/advs.202500311

Figure Lengend Snippet: CTGF‐LYTAC inhibits TNBC proliferation and migration in vitro. a–d) Kinetic curves of the proliferation and migration of BT549 and MDA‐MB‐231 cells after CL8‐M6P 3 treatment, as assessed by xCELLigence RTCA‐DP ( n = 3). The P values were calculated by two‐way ANOVA. Representative images and statistics of transwell migration assays of e–g) BT549 and MDA‐MB‐231 cells following CL8‐M6P 3 treatment and of h–j) BT549 and MDA‐MB‐231 cells (CTGF‐knockdown) treated with CTGF in the absence and presence of CL8‐M6P 3 ( n = 3). The P values were calculated by one‐way ANOVA. Top 10 KEGG signaling pathways of transcriptomics in BT549 cells after k) CTGF knockdown and l) CL8‐M6P 3 treatment, and that of proteomics in BT549 after m) CL8‐M6P 3 treatment. n) Venn plot of KEGG signaling pathways in (k‐m). o) Protein–protein interactions (PPI) of CTGF by GeneMANIA. p–t) CL8‐M6P 3 treatment reduced levels of p‐Smad3, Notch3, Jagged1 of TGF‐β signaling pathway in BT549 and MDA‐MB‐231 cells after 12 h ( n = 3). The P values were calculated by one‐way ANOVA. For (a–d,f,g,i,j), the quantified data from different experiments were presented as the mean ± SD.

Article Snippet: The PVDF membrane was blocked in TBST containing 5% BSA, and then incubated with primary antibodies, including CTGF Rabbit mAb (D8Z8U, CST), Smad3 Rabbit mAb (C67H9, CST), Phospho‐SMAD3 (Ser423/425) Rabbit mAb (C25A9, CST) Notch3 Rabbit mAb (D11B8, CST), Jagged1 Rabbit mAb (D4Y1R, CST), His‐Tag Rabbit mAb (D3I1O, CST), β‐tubulin mouse monoclonal antibody (LF203, Epizyme Biotech), and GAPDH mouse monoclonal antibody (LF205, Epizyme Biotech), overnight at 4 °C.

Techniques: Migration, In Vitro, Knockdown, Protein-Protein interactions